H2A.Z knockdown RNAseq. Mus musculus

This experiment seeks to ascertain the transcriptional changes in the adult mouse hippocampus (CA1 subregion) that occur following viral knockdown of the histone variant H2A.Z. We are especially interested in understanding the role of this histone variant in memory formation and memory maintenance in the adult central nervous system. Overall design: This experiment includes 3 groups, each with 3 biological replicates. Samples S108, S109, and S110 are from controls infected with an AAV expressing a scrambled shRNA control. Samples A100, A101, A102, A104, A106, and A107 were infected with an AAV expressing an shRNA against H2A.Z. Samples A100, A101, and A102 were naive animals, whereas samples A104, A106, and A107 were trained in contextual fear conditioning.

本实验旨在探明成年小鼠海马体CA1亚区在组蛋白变体H2A.Z经病毒介导敲低后发生的转录水平变化。我们尤为关注该组蛋白变体在成年中枢神经系统记忆形成与记忆维持过程中的作用。实验整体设计：本实验共设置3组，每组包含3个生物学重复。样本S108、S109、S110为对照组，均感染了表达乱序短发夹RNA（shRNA）对照的腺相关病毒（AAV）。样本A100、A101、A102、A104、A106及A107均感染了靶向H2A.Z的短发夹RNA（shRNA）表达腺相关病毒（AAV）。其中，样本A100、A101、A102为未经过训练的动物，而样本A104、A106、A107则接受了情境恐惧条件反射训练。