Gut microbiota in human with different TCM constitutions.

Fecal microbiota transplantation (FMT) has been tested for the prevention and treatment of various extra-intestinal diseases, but its efficacy is not stable, which is related to the unclear mechanism and the lack of individualized matching donors. In addition, the high cost and low efficiency of donor screening have also become obstacles to the clinical application of FMT. In this study, college students were identified by traditional Chinese medicine (TCM) constitution and fecal samples of single constitution volunteers were obtained. The characteristics of gut microbiota of each constitution were analyzed. It was found that there were significant differences in gut microbiota between biased constitution and balanced constitution. Moreover, in this study, TCM constitution identification was introduced into donor screening whose results showed that volunteers with balanced constitution had a higher proportion of qualified fecal bacteria donors through various biochemical tests, and animal experiments also showed that there were significant differences in weight loss effects of FMT among different constitution donors. This study provides an important reference for the application of TCM constitution theory in improving the screening efficiency of FMT donors and the feasibility of matching donors with individualized treatment of recipients.

粪便菌群移植（Fecal microbiota transplantation，FMT）已被试用于多种肠外疾病的预防与治疗，但其疗效并不稳定，这与作用机制尚不明确、缺乏个体化匹配供体密切相关。此外，供体筛查成本高昂且效率低下，也成为了FMT临床应用的阻碍因素。本研究通过中医体质（Traditional Chinese Medicine，TCM）分型对大学生进行辨识，采集单一体质志愿者的粪便样本，分析不同体质人群的肠道菌群特征。研究发现，偏颇体质与平和体质人群的肠道菌群存在显著差异。此外，本研究将TCM体质辨识应用于供体筛查，结果显示经多项生化检测后，平和体质志愿者合格粪便菌供体的占比更高；动物实验也证实，不同体质供体的FMT减重效果存在显著差异。本研究为TCM体质理论应用于提升FMT供体筛查效率，以及实现受体个体化治疗的供体匹配可行性提供了重要参考依据。