Differential expression analysis of a Vibrio parahaemolyticus ∆luxO mutant compared to wild-type in mouse intestinal mucus. Vibrio parahaemolyticus RIMD 2210633

Vibrio parahaemolyticus is the leading bacterial cause for seafood-related gastroenteritis worldwide. As an intestinal pathogen, V. parahaemolyticus competes with other commensal bacteria for the same pool of nutrients. The major source of nutrition for intestinal bacteria is intestinal mucus. We wanted to determine the expression profile of wild-type V. parahaemolyticus in mouse intestinal mucus and then perform a differential expression analysis in a ∆luxO deletion mutant, in which the high cell density quorum sensing regulator OpaR is constitutively expressed and low cell density regulator AphA is repressed. Overall design: RNA-Seq analysis was performed on RNA isolated from two biological replicates of wild-type Vibrio parahaemolyticus strain RIMD2210633 and three biological replicates the ∆luxO mutant strain, grown in M9 minimal media supplemented with mouse intestinal mucus.

副溶血性弧菌（Vibrio parahaemolyticus）是全球范围内引发海鲜相关性胃肠炎的首要细菌性致病菌。作为一种肠道致病菌，副溶血性弧菌会与其他共生细菌竞争同一营养池中的营养物质。肠道细菌的主要营养来源为肠黏液。本研究旨在明确野生型副溶血性弧菌在小鼠肠黏液中的表达谱，并对ΔluxO缺失突变株开展差异表达分析：该突变株中高细胞密度群体感应（quorum sensing）调节因子OpaR呈组成型表达，低细胞密度调节因子AphA则受到抑制。整体实验设计：对在添加了小鼠肠黏液的M9基础培养基（M9 minimal media）中培养的野生型副溶血性弧菌菌株RIMD2210633的2个生物学重复样本，以及ΔluxO突变菌株的3个生物学重复样本所提取的RNA进行RNA-Seq分析。