Next Generation Sequencing analysis using splenic DCs freshly isolated from Wild Type and TBK1-DKO mice. Mus musculus

In this experiment, we analyzed the gene expression profile of WT and TBK1-deficient splenic DCs by RNA sequencing based on three independent samples. The results revealed significant alterations in the expression of a number of genes, most notably the enhanced expression of a large subset of IFN-responsive genes in the TBK1-deficient DCs. Overall design: Fresh splenic DC mRNA profiles of 8-week old wild type (WT) and TBK1-DKO mice were generated by deep sequencing, in triplicate

本实验以三份独立生物学样本为基础，通过RNA测序（RNA sequencing）分析了野生型（WT）与TBK1缺陷型脾脏树突状细胞（splenic DCs）的基因表达谱。结果显示，大量基因的表达发生显著改变，其中TBK1缺陷型脾脏树突状细胞内干扰素应答基因（IFN-responsive genes）的大型亚群表达上调最为突出。整体实验设计：提取8周龄野生型（WT）与TBK1双敲除（TBK1-DKO）小鼠的新鲜脾脏树突状细胞mRNA，进行深度测序（deep sequencing），每组设置三次生物学重复。