COS7 CHMP4A+VPS4DN
收藏Mendeley Data2024-01-31 更新2024-06-27 收录
下载链接:
https://data.caltech.edu/doi/10.22002/rcgag-6bg55
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资源简介:
Tilt Series Date: 2011-12-22 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: imod Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine Sample Preparation: manual blotting
倾斜序列采集日期:2011-12-22
数据采集者:科拉·伍德沃德(Cora Woodward)
物种/标本:非洲绿猴(Cercopithecus aethiops,African green monkey)
细胞株:COS-7,ATCC保藏编号CRL-1651
倾斜序列采集参数:单轴倾斜模式,倾斜范围为(-60.0°, 60.0°),步长1°,采用恒定角度增量,电子辐照剂量为150.0 eV/Ų,欠焦量为-6.0 μm,放大倍数为22500倍
采集软件:UCSF tomo
上传方式:rundir
所用数据处理软件:imod
合作者及职责分工:表达载体由韦斯·桑德奎斯特(Wes Sundquist)提供;细胞转染、冷冻及成像工作由科拉·伍德沃德(Cora Woodward)在加州理工学院(Caltech)完成
纯化、培养条件及处理方式:将完整细胞培养于电镜载网表面,并通过lipofectamine(脂质体转染试剂)完成转染
样品制备流程:手动滤纸吸附(blotting)
创建时间:
2024-01-31



