EEL human visual cortex 445 genes RNA data
收藏DataCite Commons2022-09-20 更新2024-08-18 收录
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https://figshare.com/articles/dataset/EEL_human_visual_cortex_445_genes_RNA_data/20324865/1
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资源简介:
<strong>Raw RNA locations of a visual cortex human brain section produced by EEL FISH for 445 genes.</strong> <br> RNA files are in the .parquet format which can be opened with FISHscale (https://github.com/linnarsson-lab/FISHscale) or any other parquet file reader (https://arrow.apache.org/docs/index.html) <br> <strong>RNA .parquet files</strong> Position and gene label for all RNA molecules. "c_px_microscope_stitched" contains X coordinates. "r_px_microscope_stitched" contians Y coordinates. The unit are pixels with a size of 0.18 micrometer. Multiply by 0.18 to get um scale. <br> "hamming_distance" is the hamming distance divided by the barcode length (16). The closer to 0 the better the barcode. <br> <strong>Gene colors .pkl file</strong> Pickled Python dictionary with gene colors used in the paper.
**本数据集包含通过EEL FISH技术获取的人类大脑视觉皮层切片中445个基因的原始RNA定位信息。**
RNA数据文件采用.parquet格式,可通过FISHscale工具(https://github.com/linnarsson-lab/FISHscale)或任意parquet文件阅读器(https://arrow.apache.org/docs/index.html)打开
**RNA .parquet文件**:包含所有RNA分子的位置与基因标签信息。其中"c_px_microscope_stitched"字段存储X坐标,"r_px_microscope_stitched"字段存储Y坐标,原始单位为像素,单个像素尺寸为0.18微米,将坐标值乘以0.18即可转换为微米尺度。
"hamming_distance"字段为经条形码长度(16)归一化后的汉明距离,其值越接近0,代表条形码匹配质量越好。
**基因颜色.pkl文件**:存储论文中使用的基因颜色信息的Python pickle序列化字典。
提供机构:
figshare
创建时间:
2022-07-16



