Small RNA Sequencing facilitates quantitative analysis of miRNA expression in feces from mice during DSS-induced colitis and the recovery phase

Mice were exposed to 2.5% DSS (MW 36,000–50,000, MP Biomedicals) prepared in autoclaved drinking water for five days, after which they were provided DSS-free drinking water for 13 days. On day 12, 16.7% of mice (20 of 120) whose body weight returned to the same level as on day 1 (the change of body weight was less than 0.5 g) and exhibited an DAI of 0 or 1.00, were used as “well-recovered” mice. To identify which fecal miRNAs were affected during DSS-induced colitis and the recovery phase, we performed small RNA sequencing in feces obtained from well-recovered mice on days 0, 5, and 12 of the treatment regimen. Overall design: Fecal miRNA profiles of mice before inflammation (n=4), acute inflammatory phase (n=3), and the recovery phase (n=4) were generated by Small RNA Sequencing

将小鼠暴露于2.5%葡聚糖硫酸钠（Dextran Sulfate Sodium, DSS）配制的高压灭菌饮用水中，该DSS分子量为36000~50000，购自MP Biomedicals公司，持续造模5天；随后更换为不含DSS的饮用水，继续饲养13天。在造模第12天时，选取体重恢复至第1天基线水平（体重变化量小于0.5g）且疾病活动指数（Disease Activity Index, DAI）为0或1.00的小鼠（120只中共计20只），作为“完全恢复”小鼠。为探究DSS诱导结肠炎及恢复阶段中粪便microRNA（miRNA）的表达变化，我们对该治疗方案第0、5、12天的完全恢复小鼠的粪便样本进行了小RNA测序。实验设计概况：通过小RNA测序获得了炎症前（n=4）、急性炎症期（n=3）及恢复阶段（n=4）小鼠的粪便miRNA表达谱。