Molecular profile of ketamine in comparison to memantine and phencyclidyne and other psychotropic drugs [mouse striatum]. Mus musculus

Ketamine has been found to elicit a rapid antidepressant effects in treatment-refractory affective disorders. To indicate the underlying mechanism of action we have performed whole-genome microarray profiling. Moreover, the effects of ketamine were compared to other NMDA receptor antagonists phencyclidine and memantine. Type: Drug response, Time-course, Gene expression profiling with Illumina Microarrays Keywords: Ketamine, NMDA antagonist, Phencyclidyne, Memantine, Time-course, Gene Expression, Acute treatment Overall design: The microarray experiment was performed to analyze time-course of drug-induced transcriptional response in C57BL/6J mouse striatum. Three NMDA antagonists (ketamine 20 mg/kg, phencyclidine 5 mg/kg and memantine 15 mg/kg i.p.) were selected for the comparison. Drug doses were based on the literature. To analyze dynamics of early, intermediate and relatively late changes of mRNA abundance the experiment was performed in four time points (1, 2, 4 and 8h after drug administration). To exclude influence of drug injection and circadian rhythm on gene expression profile, control groups of saline treated and naive animals were prepared for each time point. Samples from 2 mice were pooled per microarray, 3 biological replicates were used per time point and 12 arrays per each drug. To provide appropriate balance in the whole dataset groups were equally divided between the array hybridization batches. 'Complete' normalized data and non-normalized data (containing control rows not represented in Platform GPL6105) are linked below as supplementary files.

氯胺酮（Ketamine）已被证实可在难治性情感障碍患者中产生快速抗抑郁效应。为阐明其潜在的作用机制，本研究开展了全基因组微阵列谱分析。此外，还将氯胺酮的效应与其他NMDA受体拮抗剂——苯环利定（Phencyclidine）和美金刚（Memantine）进行了对比。
数据集类型：药物响应、时间序列分析、基于Illumina微阵列（Illumina Microarrays）的基因表达谱分析
关键词：氯胺酮、NMDA受体拮抗剂、苯环利定、美金刚、时间序列、基因表达、急性给药
实验设计：本微阵列实验旨在分析C57BL/6J小鼠纹状体中药物诱导的转录响应的时间动态。本研究选取3种NMDA受体拮抗剂（氯胺酮20 mg/kg、苯环利定5 mg/kg、美金刚15 mg/kg，腹腔注射）开展对比实验，药物剂量均参考已有文献确定。为解析mRNA丰度的早期、中期及相对晚期变化，实验设置了给药后1、2、4、8 h共4个时间点。为排除药物注射操作与昼夜节律对基因表达谱的干扰，针对每个时间点均设置了生理盐水处理对照组与未处理空白对照组。每个微阵列样本混合2只小鼠的组织样本，每个时间点设置3次生物学重复，每种药物对应12张微阵列芯片。为确保整个数据集的均衡性，所有实验组均按芯片杂交批次进行均匀分组。完整的标准化数据与非标准化数据（包含平台GPL6105中未覆盖的对照探针行）已作为补充文件附于下方。