Gene expression data from mouse ES cell-derived primitive NSCs and definitive NSCs. Mus musculus

Primitive neural stem cells (NSCs) could be derived from pluripotent mouse embryonic stem (ES) cells, and then differentiate into definitive-type neural stem cells which resemble NSCs obtained from the central nervous system. Hence, primitive NSCs define an early stage of neural induction and provide a model to understand the mechanism that controls initial neural commitment. In this study, we performed microarray assay to analyze the global transcriptional profiles in mouse ES cell-derived primitive and definitive NSCs and to depict the molecular changes during the multi-staged neural differentiation process. Overall design: Primitive NSCs derived directly from ESCs in Lif (p-NSC_L), primitive NSCs that were sub-cultured in the presence of Lif and FGF (p-NSC_LF), as well as definitive NSCs derived from primitive NSCs in medium containing FGF and EGF, were collected for RNA extraction and hybridization on Affymetrix microarrays. Mouse ESCs and NSCs obtained from mouse embryonic brain (E11.5) were included for controls. For each cell type, we collected two biological replicate samples for microarray analysis.

原始神经干细胞（neural stem cells，NSCs）可由多能小鼠胚胎干细胞（embryonic stem cells，ES细胞）诱导获得，后续可分化为与中枢神经系统来源NSCs表型一致的定型型神经干细胞。因此，原始NSCs代表神经诱导的早期阶段，可为解析调控初始神经谱系定型的分子机制提供理想研究模型。本研究通过基因芯片检测，分析了小鼠ES细胞来源的原始及定型型NSCs的全转录组表达谱，以刻画多阶段神经分化过程中的分子动态变化。总体实验设计：本研究收集三类细胞样本用于RNA提取及Affymetrix基因芯片杂交：直接于白血病抑制因子（Leukemia Inhibitory Factor，LIF）培养体系中由ES细胞诱导获得的原始NSCs（p-NSC_L）、在LIF与成纤维细胞生长因子（Fibroblast Growth Factor，FGF）共同培养体系中继代培养的原始NSCs（p-NSC_LF），以及在含FGF与表皮生长因子（Epidermal Growth Factor，EGF）的培养基中由原始NSCs诱导获得的定型型NSCs。本研究同时纳入小鼠ES细胞及小鼠胚胎脑（E11.5）来源的NSCs作为对照。每种细胞类型均设置2个生物学重复样本用于基因芯片分析。