m6A-seq analysis of Wild Type and Mettl3-/- BMDMs (bone-marrow-derived macrophages) Transcriptomes

To investigate the role of METTL3-mediated m6A modification in macrophage, we performed m6A-sequencing to map the m6A modification in bone-marrow-derived macrophages (BMDMs) in wild type (WT) and Mettl3-/- mice. Overall design: m6A-seq profiles of BMDMs in 8 weeks wild type (WT) and Mettl3-/- (KO) mice were generated by deep sequencing, with biologic replicates.

为探究甲基转移酶样3（METTL3）介导的N6-甲基腺嘌呤（m6A）修饰在巨噬细胞中的作用，本研究通过m6A测序绘制了野生型（WT）及Mettl3-/-小鼠的骨髓源性巨噬细胞（BMDMs）中的m6A修饰图谱。
实验设计概述：本研究通过深度测序结合生物学重复，获取了8周龄野生型（WT）与Mettl3-/-（KO）小鼠骨髓源性巨噬细胞的m6A测序图谱。