Online resources: Portuguese Pinus nigra populations: genetic diversity, structure and relationships inferred by SSR markers

<b>Online resources 1</b>: SSR database obtained by thirteen SSR markers for six populations of Pinus nigra in Portugal. The populations are representative of the species distribution in Portugal: Manteigas and Vale do Zêzere (center), Campeã and Vila Pouca de Aguiar (north) and Paredes de Coura and Caminha (northwest).<b>Online resources 2</b>: Genetic diversity statistics assayed per Portuguese P. nigra population and SSR locus. Notes: na - observed number of alleles; ne - effective number of alleles (Kimura and Crow 1964); I - Shannon’s Information Index (Lewontin 1972); h – Nei’s gene diversity index (Nei 1973); Ho – observed heterozygosity; He – expected heterozygosity (Levene 1949); the F – fixation index; and s.d. – standard deviation.<b>Online resources 3</b>: Genetic differentiation coefficient (Gst) and differentiation index of allele frequencies (Jost’s D) estimated per SSR locus, and total mean value (± standard deviation, s.d.) determined with the 13 SSR markers.<b>Online resources 4</b>: PCoA based on the pooled SSR data using the Nei’s genetic distance coefficient (Nei 1978), showing the projection of the six Portuguese P. nigra populations apart from the seven foreign samples representative of the subspecies dalmatica, pallasiana, laricio, nigra and salzmannii. The cumulative percentage of total variation explained by the first three coordinates was 74.50%.

<b>在线资源1</b>：针对葡萄牙境内6个欧洲黑松（Pinus nigra）种群，通过13个简单序列重复（Simple Sequence Repeat，SSR）标记构建的SSR数据库。该6个种群代表了该物种在葡萄牙的分布范围：曼特伊加斯与泽泽雷谷（中部种群）、坎佩昂与维拉波萨德阿吉亚尔（北部种群）以及帕雷什德库拉与卡米尼亚（西北部种群）。
<b>在线资源2</b>：针对葡萄牙境内欧洲黑松种群及SSR位点所测定的遗传多样性统计量。注：na为观测等位基因数；ne为有效等位基因数（Kimura与Crow，1964）；I为香农信息指数（Lewontin，1972）；h为奈伊基因多样性指数（Nei，1973）；Ho为观测杂合度；He为期望杂合度（Levene，1949）；F为固定指数；s.d.为标准差（standard deviation）。
<b>在线资源3</b>：针对每个SSR位点估算的遗传分化系数（Gst）与等位基因频率分化指数（乔斯特D指数，Jost’s D），以及基于13个SSR标记计算得到的总体均值（±标准差，s.d.）。
<b>在线资源4</b>：基于合并SSR数据、采用奈伊遗传距离系数（Nei，1978）完成的主坐标分析（Principal Coordinates Analysis，PCoA），展示了6个葡萄牙欧洲黑松种群与7个代表达尔马提亚亚种、帕拉西阿纳亚种、拉里乔亚种、黑松亚种以及萨尔茨曼尼亚种的外来样本的分布投影。前3个坐标累计解释了74.50%的总变异。