Single-nucleus transcriptomic sequencing of aging hypothalamus treated with 17a-estradiol

To dissect the role of 17a-estradiol in lifespan extending and its potential side effects for long-term administration, the pooled hypothalami from aging male Norway brown rats treated with 17a-estradiol for 6 months (O.T), aged male control (O), and young male control (Y) were subjected to single-nucleus transcriptomic sequencing (snRNA-seq).为解析17α-雌二醇（17α-estradiol）在寿命延长中的作用及其长期给药的潜在不良反应，本研究对三组样本的混合下丘脑组织进行了单细胞核转录组测序（snRNA-seq）：三组样本分别为经17α-雌二醇给药6个月的衰老雄性挪威棕鼠（O.T组）、衰老雄性空白对照组（O组）以及年轻雄性空白对照组（Y组）。