RNAseq analyses of pathogenic Burkholderia glumae under in vitro and in vivo conditions

We analyzed B. glumae transcriptome from infected rice tissue using an RNAseq technique. To identify unique expression of B. glumae genes within rice tissues, we compared in vivo transcriptome data of B. glumae to in vitro data. To accomplish this, we analyzed differentially expressed genes (DEGs) and identified 2,906 transcripts that were significantly altered. Overall design: Three in vitro (culture) and three in vivo (in plant) RNAseqs in B. glumae本研究采用RNA测序（RNAseq）技术，对感染水稻组织中的谷泛菌（B. glumae）转录组展开分析。为明确该菌在水稻组织内的基因特异性表达特征，我们将其体内转录组数据与体外培养的转录组数据进行了对照分析。为完成该研究目标，我们对差异表达基因（DEGs）进行了分析，最终鉴定出2906个发生显著表达变化的转录本。实验整体设计：针对谷泛菌设置3组体外（培养体系）与3组体内（植株内）RNA测序样本。