Nucleoside quantification in the ventral midbrain of G2019S LRRK2 knockin mice

This Zenodo deposit contains a publicly available description of the Dataset: Title: "Nucleoside quantification in the ventral midbrain of G2019S LRRK2 knockin mice". Description: G2019S LRRK2 knockin mice and their wild-type littermates (3-5 months old) were anesthetized with an isoflurane vaporizer, tissue were then collected and immediately snap frozen in liquid nitrogen. Frozen tissues were cryopulverized, homogenized and processed using the Bligh-Dyer method. Absolute nucleoside quantitation was accomplished by running an external calibration curve with the samples. The stock mix contained Deoxyadenosine (dA), Deoxycytidine (dC), Deoxyguanosine (dG), Deoxythymidine (dT), and Deoxyuridine (dU). Targeted metabolomics peak picking and integration were conducted in Skyline (v25.1) using accurate mass MS1, MS2 fragmentation pattern matching, and retention time derived from analytical standards run through each chromatography method. Raw data files for all samples of a given experiment were imported and metabolite peaks were auto-integrated based standard verified m/z, precursor adducts, and retention times for all metabolites of interest. Each experimental group contained biological replicates (n = 9 per group). This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset本Zenodo存档包含一份公开可用的数据集说明，标题为「G2019S LRRK2敲入小鼠腹侧中脑的核苷定量分析」。 G2019S LRRK2敲入小鼠及其野生型同窝仔鼠（3~5月龄）经异氟烷麻醉机麻醉后，采集组织并立即置入液氮中快速冷冻。将冷冻组织进行低温粉碎、匀浆，采用布利格-戴尔（Bligh-Dyer）法进行样品前处理。通过构建外标校准曲线完成绝对核苷定量，标准储备液包含脱氧腺苷（Deoxyadenosine, dA）、脱氧胞苷（Deoxycytidine, dC）、脱氧鸟苷（Deoxyguanosine, dG）、脱氧胸苷（Deoxythymidine, dT）及脱氧尿苷（Deoxyuridine, dU）。靶向代谢组学的峰识别与积分工作在Skyline（v25.1）软件中完成，基于精确质量MS1、MS2碎片匹配模式，以及通过各色谱方法分析的分析标准品得到的保留时间进行匹配。将单次实验所有样本的原始数据文件导入后，基于经标准验证的目标代谢物的m/z值、前体加合物及保留时间自动积分代谢物峰。每组实验设置生物学重复（每组n=9）。 本数据集通过ASAP CRN云平台（cloud.parkinsonsroadmap.org）向研究人员开放，获取访问权限的操作指南可参考用户手册。 本研究由Aligning Science Across Parkinson's协作研究网络（ASAP CRN）通过迈克尔·J·福克斯帕金森症研究基金会（MJFF）资助。 本Zenodo存档由ASAP CRN云平台工作人员代表数据集作者创建，可为CRN云平台数据集提供可引用的参考文献。