Figure 2. UL12.5 is required for Phase I lytic gene expression during HSV-1 reactivation. UL12.5 is required for Phase I lytic gene expression during HSV-1 reactivation

2B. Quantification of the relative viral genome (vDNA - ICP8) in latently infected (0 hours) and reactivated (60 hours) neurons infected with either KOS-SPA and KOS-UL98 measured by qPCR .2C. Relative expression of lytic mRNAs and LAT in neurons latently infected (0 hours post reactivation) with either KOS-SPA and KOS-UL98 measured by RT-qPCR for ICP27 (IE), UL30 (E), VP16 (L), and gC (L).2D. Relative expression of lytic mRNAs (ICP27;UL30;VP16;gC) at 18 hours post-reactivation (Phase I).2E. Relative expression of lytic mRNAs (ICP27;UL30;VP16;gC) at 48 hours post-reactivation (Phase II).2F. Relative expression of ICP27 mRNA during Phase I reactivation in neurons latently infected with F/L or the rescue virus; F/LR.2G. Relative expression of ICP27 mRNA during Phase I reactivation in neurons latently infected with AN-1 or the wild-type strain KOS.

2B. 采用定量聚合酶链反应（quantitative PCR, qPCR）测定分别感染KOS-SPA与KOS-UL98的潜伏感染（再激活0小时）与再激活（再激活60小时）神经元内相对病毒DNA（viral DNA, vDNA - ICP8）水平。
2C. 采用逆转录定量聚合酶链反应（reverse transcription quantitative PCR, RT-qPCR）检测分别感染KOS-SPA与KOS-UL98的潜伏感染神经元（再激活0小时）中裂解期mRNA与潜伏相关转录本（latency-associated transcript, LAT）的相对表达量，检测靶标包括ICP27（immediate early, IE）、UL30（early, E）、VP16（late, L）与gC（late, L）。
2D. 再激活后18小时（I期）时裂解期mRNA（ICP27;UL30;VP16;gC）的相对表达水平。
2E. 再激活后48小时（II期）时裂解期mRNA（ICP27;UL30;VP16;gC）的相对表达水平。
2F. 分别感染F/L或其回复突变病毒F/LR的潜伏感染神经元在I期再激活过程中ICP27 mRNA的相对表达水平。
2G. 分别感染AN-1或野生型毒株KOS的潜伏感染神经元在I期再激活过程中ICP27 mRNA的相对表达水平。