Data used for article "Valorising feather keratin: antioxidant activity, cytotoxicity and mutagenicity during processing and gastrointestinal digestion"

The dataset contains data regarding chicken feather keratin isolate (KI) obtained by reductive extraction with L-cysteine, its enzymatic hydrolysates produced by trypsin (KI-T), chymotrypsin (KI-C), pepsin (KI-P) and subtilisin (KI-S), Maillard reaction products (MRPs) derived from KI-S and glucose (KI-S-G1 to KI-S-G9), MRPs derived from KI-S and xylose (KI-S-X1 to KI-S-X9), and data from simulated gastrointestinal digestion (SGID) of selected samples (KI, KI-S, KI-S-X4). The dataset includes data from high performance size exclusion chromatography (HP-SEC, molecular weight distribution and antiradical activity profiles), data used for determining the degree of glycation (DG) and browning intensity (A294 and A420) of MRPs, free amino acid profiles of the samples post-SGID, data used to calculate total antioxidant activity using ABTS scavenging, Fe2+ chelating and Folin-Ciocâlteu reagent reducing assays, data from cytotoxicity (MTT test in Caco-2 cells), and data from direct and indirect mutagenicity assays (Ames test in Salmonella Typhimurium TA98 and TA 100).
This work was supported by the Polish national research budget, under the National Science Centre grant number DEC-2021/41/N/NZ9/04466 and Irish Department of Agriculture, Food & the Marine DAFM-2019R495-INFOTECH.

本数据集涵盖以下多类实验数据：通过L-半胱氨酸还原提取得到的鸡羽毛角蛋白分离物（KI）；经胰蛋白酶（KI-T）、胰凝乳蛋白酶（KI-C）、胃蛋白酶（KI-P）与枯草杆菌蛋白酶（KI-S）酶解KI得到的酶解产物；由KI-S与葡萄糖反应生成的美拉德反应产物（MRPs，KI-S-G1至KI-S-G9）；由KI-S与木糖反应生成的美拉德反应产物（MRPs，KI-S-X1至KI-S-X9）；以及选定样品（KI、KI-S、KI-S-X4）的模拟胃肠道消化（SGID）相关数据。
本数据集还包含以下检测数据：高效体积排阻色谱（HP-SEC）相关数据（涵盖分子量分布与抗自由基活性谱图）；用于测定美拉德反应产物糖基化程度（DG）与褐变强度（A294和A420）的相关数据；模拟胃肠道消化后样品的游离氨基酸谱数据；基于ABTS自由基清除法、Fe²+螯合法与福林-酚（Folin-Ciocâlteu）还原法测定总抗氧化活性的相关数据；Caco-2细胞MTT细胞毒性实验数据；以及鼠伤寒沙门氏菌TA98与TA100菌株的埃姆斯（Ames）直接/间接致突变性试验数据。
本研究受波兰国家研究预算资助，依托波兰国家科学中心编号为DEC-2021/41/N/NZ9/04466的拨款项目，以及爱尔兰农业、食品与海洋部编号为DAFM-2019R495-INFOTECH的拨款项目完成。