Transcriptomic profiles of Pseudomonas aeruginosa PAO1 grown in the presence or absence of mucins or mucin-glycans

We use high-throughput sequencing to profile the response of the opportunistic mucosal pathogen Pseudomonas aeruginosa to mucins and mucin-glycans from the mucosal niche. We find that P. aeruginosa undergoes a genome-wide phenotypic shift in response to mucins and their attached glycans. Specifically, nearly all virulence pathways are downregulated in response to these host-produced factors. This study provides a framework for understanding how the host environment regulates bacterial function. Examination of differential gene expression in Pseudomonas aeruginosa in response to mucin and mucin sugars

本研究采用高通量测序技术，对机会性黏膜病原体铜绿假单胞菌（Pseudomonas aeruginosa）响应黏膜微生态位来源的黏蛋白（mucins）与黏蛋白聚糖（mucin-glycans）的转录应答特征进行了表征分析。研究结果显示，铜绿假单胞菌在黏蛋白及其结合聚糖的刺激下发生了全基因组范围的表型转变；具体表现为，几乎所有毒力通路（virulence pathways）均在这类宿主源性因子的作用下呈现表达下调趋势。本研究为解析宿主环境调控细菌功能的内在机制提供了系统性研究框架。本研究同时开展了针对铜绿假单胞菌响应黏蛋白及黏蛋白糖类的差异基因表达分析。