IL-17A and IL-6 -stimulated expression data from primary epithelial cell cultures derived from human trachea tissues. Homo sapiens

RNAs were isolated from primary cultures after 24 hour treatment with IL-17A or IL-6 (10 ng/ml) in primary human TBE cells. We used microarrays to analyze the global gene expression profiling of IL-17A or IL-6 regulated genes in TBE cells. Overall design: TBE cells were grown and stimutaed with the cytokines indicated. Total RNA were then extracted for Affymetrix microarray studies.

本研究从经白细胞介素17A（IL-17A）或白细胞介素6（IL-6，终浓度10 ng/ml）处理24小时的原代人TBE细胞原代培养体系中分离总RNA。采用微阵列（microarrays）技术分析TBE细胞中受IL-17A或IL-6调控的基因的全局基因表达谱。实验整体设计：将TBE细胞培养后，以指定细胞因子进行刺激，随后提取总RNA用于Affymetrix微阵列实验分析。