Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [indels]. Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [indels]

Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: Characterization of indels following Cas9-induced breaks in selected amplicons containing a SNP in the protospacer.

本研究开发了BreakTag方法，这是一种通用、高度并行且具备切割事件感知能力的分析方法，用于对Cas9诱导的DNA双链断裂（double strand break, DSB）进行谱分析，以鉴定影响Cas9切割的分子决定因素。
整体实验设计：对选定的、在原间隔序列中包含单核苷酸多态性（Single Nucleotide Polymorphism, SNP）的扩增子内，Cas9诱导断裂后产生的插入缺失变异（insertions-deletions, indels）开展表征分析。