Comparison of microarray expression data from 22 day mouse germ cells from control and Mgat1 conditional knockout mice

Mechanistic insights into MGAT1 loss during spermatogenesis were investigated in germ cells from 22 day males. Gene expression changes induced by deletion of Mgat 1in spermatogonia were determined using the Affymetrix GeneChip Whole Transcript Plus Reagent Kit. We include the expression data obtained from control and conditional knock out Mgat1 mutant mouse testis germ cells . We identified the genes that were differentially expressed (DEGs) in 22 day Mgat1 mutant versus control germ cells, with a significance level of ANOVA p < 0.05 and an absolute fold-change (linear) less than -2 and greater than 2.0. RNA from 3 control and 3 mutant germ cell preparations was purified using RNeasy mini kit and Rneasy Minielute clean up kit (Qiagen), RNA integrity was measured and samples with RIN >9.0 were submitted for cDNA preparation and microarray analysis.

本研究以22日龄雄性小鼠的生殖细胞为实验材料，探究精子发生过程中MGAT1缺失的分子机制。采用Affymetrix GeneChip全转录组扩增试剂试剂盒，检测精原细胞中Mgat1缺失诱导的基因表达变化。本数据集包含野生型对照与条件性敲除Mgat1的突变型小鼠睾丸生殖细胞的表达谱数据。本研究以方差分析（Analysis of Variance, ANOVA）检验P值<0.05、线性倍数变化的绝对值>2（即倍数变化<-2或>2）为筛选标准，鉴定出22日龄Mgat1突变型与对照生殖细胞间的差异表达基因（Differentially Expressed Genes, DEGs）。本研究使用Qiagen（凯杰）公司的RNeasy迷你试剂盒与RNeasy Minielute纯化试剂盒，对3份对照样本与3份突变样本的生殖细胞总RNA进行纯化；通过检测RNA完整性，选取RNA完整性数（RNA Integrity Number, RIN）>9.0的样本进行cDNA制备与微阵列分析。