Transcriptomics was used to reveal the effect of exogenous nitric nitrogen on cucumber roots under low temperature stress (PRJCA042602)

This study aimed to elucidate the molecular mechanisms by which exogenous nitrate nitrogen modulates low-temperature tolerance in cucumber (Cucumis sativus L. Xintaimici) roots. Hydroponically grown seedlings were subjected to low-temperature stress (10/15 deg C) and three NO3--N treatments: 0 mM (Group A), 12 mM (Group B), and 24 mM (Group C). Root samples were collected at 4 and 12 days post-treatment for transcriptomic (RNA-Seq and qRT-PCR) and physiological analyses.

本研究旨在阐明外源硝态氮调控‘新泰密刺’黄瓜（Cucumis sativus L. Xintaimici）根系低温耐受性的分子机制。以水培培育的黄瓜幼苗为试材，设置10/15℃低温胁迫处理与3组硝态氮（NO3--N）浓度梯度处理：0 mM（A组）、12 mM（B组）及24 mM（C组）。分别于处理后第4天与第12天采集根系样本，开展转录组测序（RNA-Seq）、实时荧光定量PCR（qRT-PCR）分析及生理指标检测。