Plasmodium falciparum intraerythrocytic stage transcription time course for NF54 and isogenic mutant line with altered K13 expression I.. Plasmodium falciparum intraerythrocytic stage transcription time course for NF54 and isogenic mutant line with altered K13 expression I.

Plasmodium falciparum intraerythrocytic stage transcription four hour time course for NF54 and PB58 (an isogenic line with a piggyBac transposon in the 5’ UTR of K13 of K13 that alters K13 expression). Overall design: The parasite strains NF54 and PB58 (the K13 mutant) were maintained in identical standard culture conditions and synchronized by 3 rounds of sorbitol synchronization. A four hour time-course was collected at 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, and 46 hours post invasion (hpi) with triplicate samples at 6, 26 and 38 hpi.

本数据集为恶性疟原虫（Plasmodium falciparum）红细胞内期的四小时转录时间进程样本，涵盖NF54与PB58两个菌株：其中PB58为K13突变株，是在K13基因5'非翻译区（5' Untranslated Region，5' UTR）携带piggyBac转座子（piggyBac transposon）的等基因系，该转座子可改变K13基因的表达水平。
实验设计如下：将恶性疟原虫菌株NF54与PB58置于完全一致的标准培养条件中，并通过三轮山梨醇同步化处理实现虫株同步化。随后于入侵后2、6、10、14、18、22、26、30、34、38、42及46小时（hpi，即hours post invasion）采集样本，其中在入侵后6、26及38小时设置三次生物学重复样本。