Effects of KD USF2 on gene expressions in C2C12. Effects of KD USF2 on gene expressions in C2C12

USF2 has emerged as a key player in various cellular processes, including cell cycle regulation， apoptosi, metabolism, and response to cellular stress. In the context of muscle biology, the role of USF2 is not clear. To elucidate the functional significance of USF2, we conducted knockdown (KD) experiments targeting USF2 in C2C12 myoblast cells. The KD of USF2 resulted in the differential expression of a set of genes, and functional enrichment analysis of genes affected by KD of USF2 were performed. Overall design: To investiage the function of USF2 in C2C12 cells, expression profiling of genes affetcted by knocking donwn USF2 were evaluated by high throughput sequencing

USF2已成为多种细胞进程的关键调控因子，涵盖细胞周期调控、细胞凋亡、代谢及细胞应激应答等过程。在肌肉生物学领域，USF2的功能尚未明确。为阐明USF2的功能意义，我们在C2C12成肌细胞中开展了靶向USF2的基因敲低（knockdown, KD）实验。USF2基因敲低后，一组基因出现差异表达；随后我们对受USF2敲低影响的基因进行了功能富集分析。整体实验设计：为研究USF2在C2C12细胞中的功能，我们通过高通量测序对USF2敲低后受影响基因的表达谱进行了检测与评估。