Bos taurus breed:Multiple Raw sequence reads. Bos taurus breed:Multiple

The common management practice of manually removing horns from cattle is an increasing animal welfare concern for consumers. Utilizing currently available polled (hornless) genetics to create fewer horned animals would take many generations since existing polled sires are primarily heterozygous animals with lower genetic merit than horned sires. An alternative strategy is to utilize gene editing technology to incorporate the polled allele into elite genetic backgrounds. Two gene edited polled bulls were created using TALENs (Carlson et al., 2016). One of these bulls was bred to Horned Hereford cows to evaluate the transmission of the polled allele to offspring. Six calves (1 female, 5 males) were born, heterozygous for the polled allele and lacking horns. Whole genome sequence reads for these animals are included, as well as sequences for purebred Horned Hereford and Hereford x Holstein contemporary controls. The long-term goals of this project are to provide empirical data on the phenotypic and genotypic durability of the polled genome edited trait, the health and development of offspring from genome edited animals, and the composition of animal products derived from the offspring of a genome edited bull and control bulls. This research will further our understanding of any novel risks associated with the use of genome editing in livestock.

畜牧业中普遍采用的人工去除牛只牛角的管理操作，正日益引发消费者对动物福利的担忧。利用现有可获取的无角（polled）遗传学资源培育少角牛群，需历经多代选育，因当前现存的无角种公牛多为杂合子个体，其遗传性能劣于有角种公牛。另一项可选策略是借助基因编辑技术，将无角等位基因整合至优良遗传背景中。研究团队借助TALEN技术培育出两头基因编辑无角公牛（Carlson等，2016）。将其中一头公牛与有角赫里福德母牛配种，以评估无角等位基因向后代的传递情况。共计产下6头犊牛（1头雌性、5头雄性），均为无角等位基因杂合子且未生长牛角。本数据集包含上述个体的全基因组测序读段，以及纯种有角赫里福德牛与赫里福德×荷斯坦同期对照牛的基因组序列。本项目的长期目标是提供三类实证数据：一是基因编辑无角性状的表型与基因型稳定性；二是基因编辑动物后代的健康与生长发育状况；三是基因编辑公牛后代与对照公牛后代所产动物产品的成分组成。本研究将深化学界对家畜基因编辑技术应用相关潜在新型风险的认知。