Fig2B_RACE_STOML2_R2_LG292.tiff

Xrn1 knock out A549 cells were infected with WT influenza A/Puerto Rico/8/1934 virus ("PR8") or PA-X deficient PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2,YKT6, TUBA1B, BCAP31 ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.

将Xrn1基因敲除的A549细胞分别感染野生型甲型流感病毒A/波多黎各/8/1934（简称PR8）、PA-X缺陷型PR8-PA(ΔX)毒株，同时设置空白感染对照组，感染16小时后进行RNA提取。随后使用针对STOML2、YKT6、TUBA1B、BCAP31的特异性引物，在各基因的预测切割位点下游约250-300个核苷酸（nt）处开展5'末端快速扩增（5’RACE）实验，将聚合酶链式反应（PCR）产物进行琼脂糖凝胶电泳检测。