A unique population of regulatory T cells in heart potentiates cardiac protection from myocardial infarction [scRNA-seq]

Purpose: The aims of the study were to identify that heart Tregs were clonally expanded, and single-cell TCR repertoires of heart Tregs were different from spleen Tregs. Methods: CD4+ T cells from injured hearts and paired spleens of the C57BL/6J mice were double-sorted by magnetic-activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) to generate single cell RNA-sequence and paired V(D)J sequence 7 days after myocaridal infarction (MI). Results: The characteristics of clonal restriction in heart Tregs were investigated, and there were just a little TCR-sequence sharing between spleen Tregs and heart Tregs. single-cell transcriptome and clonotype of injured heart CD4+ T cells, spleen CD4+ T cells isolated from 8-week-old C57BL/6J mice 7 days post-MI

研究目的：本研究旨在明确心脏调节性T细胞（Regulatory T cells, Tregs）发生克隆扩增，且心脏调节性T细胞的单细胞T细胞受体（T cell receptor, TCR）库与脾脏调节性T细胞存在差异。
研究方法：对C57BL/6J小鼠心肌梗死（Myocardial Infarction, MI）造模后第7天的受损心脏及配对脾脏来源的CD4+ T细胞，先后通过磁激活细胞分选（Magnetic-Activated Cell Sorting, MACS）与荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）进行双重分选，以制备单细胞RNA测序及配对V(D)J序列测序样本。
研究结果：本研究解析了心脏调节性T细胞的克隆限制性特征，发现脾脏调节性T细胞与心脏调节性T细胞之间仅存在极少量的TCR序列共享；本数据集涵盖8周龄C57BL/6J小鼠心肌梗死造模后第7天的受损心脏CD4+ T细胞与脾脏CD4+ T细胞的单细胞转录组及克隆型数据。