Becoming LTi - Dataset : Single-cell RNA-seq of Embryo Fetal Liver tissue at stage 13.5 days

Dataset from article : Distinct waves from the hemogenic endothelium give rise to layered Lymphoid Tissue Inducer cell ontogeny

Summary: During embryogenesis Lymphoid Tissue Inducer (LTi) cells are essential for lymph node organogenesis. These cells are part of the Innate Lymphoid Cell (ILC) family. Although their earliest embryonic hematopoietic origin is unclear, other innate immune cells were shown to be derived from both early hemogenic endothelium in the yolk-sac as well as the aorta-gonad-mesonephros. A proper model to discriminate between these locations was unavailable. In this study, using a new Cxcr4-CreERT2 lineage tracing model, we identify a major contribution from embryonic hemogenic endothelium, but not yolk-sac, towards the LTi progenitors. Conversely, embryonic LTi cells are replaced by hematopoietic stem cell derived cells in adult. We further show that within the fetal liver common lymphoid progenitors differentiate into highly dynamic alpha-lymphoid precursor cells, which at this embryonic stage preferentially mature into LTi precursors and establish their functional LTi cell identity only after reaching the periphery.

Data :

1. SPlab_BecomingLTi_Stage13.5_FetalLiver_00_RawData : Single-cell RNA-seq data for Mouse Embryo Fetal Liver at stage 13.5. It contains the full output of CellRanger count (v3) analysis.

本文数据集源自论文：《造血内皮细胞的不同发育波次介导分层式淋巴组织诱导细胞的发育起源》
摘要：在胚胎发育阶段，淋巴组织诱导（Lymphoid Tissue Inducer, LTi）细胞对于淋巴结的器官发生过程至关重要。这类细胞隶属于先天淋巴样细胞（Innate Lymphoid Cell, ILC）家族。尽管其最早的胚胎造血起源尚未明确，但已有研究表明，其他先天免疫细胞可同时起源于卵黄囊中的早期造血内皮细胞（hemogenic endothelium）以及主动脉-性腺-中肾（aorta-gonad-mesonephros, AGM）区域。此前尚无能够有效区分这两种起源位点的合适实验模型。本研究借助全新的Cxcr4-CreERT2谱系示踪模型，证实胚胎造血内皮细胞（而非卵黄囊）对LTi前体细胞具有主要的发育贡献。与之相反，成体体内的胚胎型LTi细胞会被造血干细胞衍生的细胞所替代。本研究进一步发现，在胎肝中，普通淋巴样前体细胞会分化为具有高度动态特性的α-淋巴样前体细胞；在该胚胎阶段，这类前体细胞优先成熟为LTi前体细胞，并仅在迁移至外周组织后才确立其功能性LTi细胞身份。
数据集说明：
1. SPlab_BecomingLTi_Stage13.5_FetalLiver_00_RawData：该数据集为小鼠胚胎13.5天胎肝的单细胞RNA测序数据，包含CellRanger count（v3）分析的完整输出结果。