Ligand-dependent hedgehog signaling maintains an undifferentiated, malignant osteosarcoma phenotype. Ligand-dependent hedgehog signaling maintains an undifferentiated, malignant osteosarcoma phenotype

To determine the differentially expreesed genes in osteosarcoma cell lines MG63 and U2OS stimulated with hedgehog ligand (SHH) for 24 hours MG63 and U2OS cells were treated with PBS or 1ug/ml of recombinat human sonic hedgehog for 24 hours. RNA was isolated from the cells and RNAseq performed The results present differentially expressed genes when osteosarcoma cell lines are stimulated with SHH Overall design: mRNA profile of MG63 and U2OS cell lines treated with PBS or SHH

本研究旨在鉴定经刺猬家族配体音猬因子（sonic hedgehog, SHH）刺激24小时的骨肉瘤细胞系MG63与U2OS中的差异表达基因。实验中将MG63及U2OS细胞分别用磷酸盐缓冲液（PBS）或1μg/ml重组人音猬因子处理24小时，随后提取细胞总RNA并进行RNA测序（RNA-seq）。本研究结果呈现了骨肉瘤细胞系经SHH刺激后的差异表达基因情况。实验整体设计：经PBS或SHH处理的MG63与U2OS细胞系的mRNA表达谱。