RNA sequencing of surface mucus producing cells captured with Laser Microdissection and Pressure Catapulting in mouse stomachs infected with Helicobacter pylori Sydney Strain 1

This RNA sequencing (RNAseq) data comes from mouse strain C57/BL6 gastric surface mucus cells, also known as foveolar cells. 5 mice were non-infected controls, and 5 were infected with Helicobacter Pylori strain SS1 14 days prior to harvest. Surface mucous-producing epithelial cells were collected from the mice using laser microdissection and pressure catapulting (LMPC) with a PALM MicroBeam (Carl Zeiss, Oberkochen, Germany) at CCI (Centre for Cellular Imaging, Sahlgrenska Academy, Gothenburg, Sweden). Libraries were prepared with SMARTer Total Stranded RNA-seq, Pico input mammalian - V3 (Takara Bio, Kusatsu, Japan). The sample from one mouse in the infected group failed library prep. The remaining 9 samples were sequenced with NovaSeq 6000 (Illumina, San Diego, USA at NGI (National Genomics Infrastructure, Solna, Sweden).

本RNA测序（RNAseq）数据集取自C57/BL6品系小鼠的胃表面黏液细胞，该类细胞亦被称为胃小凹上皮细胞（foveolar cells）。实验设置5只未感染的小鼠作为空白对照组，其余5只在处死取材前14天感染幽门螺杆菌（Helicobacter Pylori）SS1菌株。研究人员在瑞典哥德堡萨尔格伦斯卡学院细胞成像中心（CCI，Centre for Cellular Imaging），使用蔡司（Carl Zeiss，德国奥伯科亨）PALM MicroBeam激光显微切割系统，通过激光显微切割联合压力弹射法（LMPC）收集小鼠的表面产黏液上皮细胞。文库构建采用Takara Bio（日本草津）SMARTer 总链特异性RNA-seq Pico输入哺乳动物样本-V3试剂盒。感染组中有1只小鼠的样本未能完成文库构建，剩余9份样本采用美国圣地亚哥Illumina公司NovaSeq 6000测序平台，在瑞典索尔纳国家基因组学基础设施中心（NGI，National Genomics Infrastructure）完成测序。