Nuclear factor of activated T-cells 5 (NFAT5) involved in gut microbial composition

The intestinal tract is frequently exposed to osmotically fluctuating environments in the lumen. The resistance of the epithelial barrier to osmotic pressure might be essential for maintaining gut homeostasis. Here, to reveal the role of osmoprotective transcription factor NFAT5 in intestinal epithelia, we examined whether NFAT5 is involved in reconstructing gut epithelial barriers and experimental colitis in mice. Moreover, we compared the gut microbial composition based on the 16S rRNA amplicon sequencing in the cecum contents and feces between wild-type and NFAT5-deficient mice to investigate the regulation of NFAT5 in the gut microbiota.

肠道内腔频繁暴露于渗透压动态波动的环境中。上皮屏障对渗透压的耐受能力或许是维持肠道稳态的必要条件。为阐明渗透保护性转录因子NFAT5在肠道上皮中的功能，本研究检测了NFAT5是否参与小鼠肠道上皮屏障的重建以及实验性结肠炎的发生。此外，本研究基于16S rRNA扩增子测序技术，对野生型与NFAT5基因缺陷型小鼠的盲肠内容物及粪便中的肠道菌群组成进行对比分析，以探究NFAT5对肠道菌群的调控作用。