Next generation sequencing facilitates quantitative analysis of wild type and overexpression of PuC3H35 transgenic lines Transcriptomes

For the RNA-seq experiments there week-old seedlings were cutting and subsequently transfer to 1/2 MS medium with or without 6% PEG6000 for 7 d and harvested roots. Samples were collected from there biological replicates (12 seedings each). RNA-seq of roots

本RNA测序（RNA-seq）实验以一周龄幼苗为材料：将幼苗切段后转接至添加或不添加6%聚乙二醇6000（PEG6000）的1/2 MS培养基中培养7天，随后收获根部组织。实验设置三次生物学重复，每个重复包含12株幼苗，采集其根部样本用于RNA测序。