From darkness to twilight: Morphological divergence between cave and surface-subterranean ecotone Niphargus species

Subterranean and surface habitats are in stark contrast in several environmental factors. Therefore, adaptation to the subterranean environment typically impedes the (re)colonisation of surface habitats. The genus Niphargus includes amphipod crustaceans that primarily occupy subterranean habitats. All its species show typical adaptations to the subterranean environment. However, some Niphargus species occur in surface-subterranean ecotones. To understand whether (i) habitat divergence is present between the cave and the ecotone species (ii) similar phenotypes emerge independently in every ecotone, we studied morphological divergence between four cave and four ecotone Niphargus species based on 13 functional morphological traits. To account for different selection acting on the sexes, we included males (N = 244) and females (N = 222). Nine out of 13 traits showed habitat- divergence. Traits related to feeding and crawling were shorter in ecotone species, while traits related to oxygenati..., , , # From darkness to twilight: Morphological divergence between cave and surface-subterranean ecotone Niphargus species

[https://doi.org/10.5061/dryad.5x69p8dc0](https://doi.org/10.5061/dryad.5x69p8dc0)

The data contains morphological measurements of 13 traits plus the body length of eight species of the *Niphargus *genus. The traits were measured on microscope slides except for body length which was measured using photos. The dataset contains the R script used for analysis.

## Description of the data and file structure

The original data file contains linear measurements of the given traits in millimeter. Species_abbreviation in the original data denote the following species: 

POD - Niphargus podpecanus; SPO - Niphargus spoeckeri; SCO - Niphargus scopicauda; STY - Niphargus stygius; TIM - Niphargus timavi; SPH - Niphargus sphagnicolus; SPI - Niphargus spinulifemur; KRA - Niphargus krameri

## Sharing/Access information

## Code/Software

The R code used for the analysis is called Bir...

地下生境与地表生境在多项环境因子上存在显著差异。因此，对地下环境的适应性通常会阻碍物种对地表生境的（再）定殖。钩虾属（Niphargus）包含主要栖息于地下生境的端足类甲壳动物（amphipod crustaceans），该属所有物种均表现出典型的地下环境适应性特征。但部分钩虾属物种分布于地表-地下生境交错带。
为明确两点：（1）洞穴物种与交错带物种间是否存在生境分化；（2）相似的表型是否在各交错带中独立出现，我们基于13项功能形态学性状，对4种洞穴钩虾与4种交错带钩虾的形态分化展开了研究。为考量不同性别所受选择压力的差异，本研究纳入了244只雄性与222只雌性个体。13项性状中有9项表现出生境分化。交错带物种的取食与爬行相关性状长度更短，而与氧合相关的性状……

# 从黑暗到微光：洞穴与地表-地下交错带钩虾属（Niphargus）物种的形态分化
https://doi.org/10.5061/dryad.5x69p8dc0

本数据集包含8种钩虾属（Niphargus）物种的13项形态学性状测量值与个体体长数据。除体长通过照片测量外，其余性状均在显微镜载玻片上完成测量。本数据集同时包含用于数据分析的R脚本。

## 数据与文件结构说明
原始数据文件包含各性状以毫米为单位的线性测量值。原始数据中的物种缩写对应如下物种：
POD - 波德钩虾（Niphargus podpecanus）；SPO - 施波克钩虾（Niphargus spoeckeri）；SCO - 尾刺钩虾（Niphargus scopicauda）；STY - 冥河钩虾（Niphargus stygius）；TIM - 蒂马维钩虾（Niphargus timavi）；SPH - 泥炭钩虾（Niphargus sphagnicolus）；SPI - 棘腿钩虾（Niphargus spinulifemur）；KRA - 克拉默钩虾（Niphargus krameri）

## 共享与获取信息

## 代码与软件
本研究使用的R脚本名为Bir……