Expression data from wild-type (WT) and lrrc19 gene knockout (KO) mice intestines. Mus musculus

LRRC19 (Leucine repeat receptor containing 19) is a newfound Toll-like receptor without definite function reported. We have detected that the lrrc19 gene had a high expression level in both human and mouse intestines and concentrated on the effect of this immune receptor in mediating the process of enteritis and intestinal cancer. To have a systematic understanding on the influence of this gene on the intracelluar environment of intestinal cell, we have conducted a microarray experiment to compare the transcriptomes of the WT and KO mice intestines under the same experimental condition. Overall design: Gut epithelial cells of WT and KO mice of the same physiological and maintaining condition were isolated and then total RNAs were extracted from them, which could reflect the respective transcription profile of WT and KO mice. These RNAs were detected by microarray and the influence of lrrc19 gene on intestine could be revealed to some extent.

LRRC19（富含亮氨酸重复序列受体19，Leucine repeat receptor containing 19）是一种新近发现、功能尚未明确的Toll样受体（Toll-like receptor）。我们检测发现，lrrc19基因在人类与小鼠肠道中均呈高表达，且本研究聚焦于该免疫受体在介导肠炎及肠癌发生发展过程中的作用。为系统解析该基因对肠道细胞胞内环境的影响，我们开展了基因芯片实验，在一致实验条件下对比野生型（WT, Wild Type）与敲除型（KO, Knock Out）小鼠肠道的转录组。总体实验设计：将处于相同生理状态与饲养条件下的野生型与敲除型小鼠肠道上皮细胞分离提取，随后从中获取总RNA，以分别反映两类小鼠的转录谱；通过基因芯片对上述RNA进行检测，可在一定程度上揭示lrrc19基因对肠道的调控影响。