Microarray analysis of mRNA and long noncoding RNA expression profiles between multiple myeloma patients and control. Microarray analysis of mRNA and long noncoding RNA expression profiles between multiple myeloma patients and control

To further investigate the differential expression gene of multiple myeloma.Three multiple myeloma samples and three normal samples were used for microarray. Overall design: Bone marrow CD138+ cells were sorted from multiple myeloma patients and normal individuals. Samples were treated with TRIzol and RNA extracted.Gene expression profiling was carried out in three multiple myeloma patients and three controls.

为深入探究多发性骨髓瘤的差异表达基因，本研究选取3份多发性骨髓瘤样本与3份正常样本开展基因微阵列（microarray）实验。实验整体设计如下：从多发性骨髓瘤患者及正常个体的骨髓中分选CD138阳性（CD138+）细胞，采用TRIzol试剂处理样本并提取RNA，最终对3份多发性骨髓瘤患者样本与3份对照样本进行基因表达谱分析。