ectomycorrhizal fungi on tree roots Targeted loci environmental. root metagenome

Rising temperatures associated with climate change have been shown to negatively affect the photosynthetic rates of boreal forest tree saplings. To quantify the responses of ectomycorrhizal (EM) fungal communities associated with poorly performing hosts, we sampled the roots of Betula papyrifera and Abies balsamea saplings growing in the B4Warmed (Boreal Forest Warming at an Ecotone in Danger) experiment. EM fungi on the root systems of both hosts were compared from ambient and +3.4°C air and soil warmed plots at two sites in northern Minnesota. EM fungal communities were assessed with high-throughput sequencing along with measures of plant photosynthesis, soil temperature, moisture, and nitrogen.

已有研究表明，气候变化引发的气温升高会对北方林（boreal forest）树苗的光合速率产生负面影响。为量化与生长弱势宿主相关的外生菌根（ectomycorrhizal, EM）真菌群落的响应特征，本研究采集了参与B4Warmed实验（危险生态交错带北方森林增温实验，Boreal Forest Warming at an Ecotone in Danger）的纸桦（Betula papyrifera）与香脂冷杉（Abies balsamea）的幼苗根系样本。本研究选取明尼苏达州北部两个试验点的原位环境样地与空气、土壤均增温3.4℃的处理样地，对比了两种宿主根系的外生菌根真菌群落；同时通过高通量测序（high-throughput sequencing）技术解析真菌群落组成，并同步测定了植物光合速率、土壤温度、土壤湿度与土壤氮素含量。