Transcription-associated Loading of Condensin II on Chromosome Arms in Embryonic Stem Cells (nocodazole ChIP-Seq)

Vertebrate condensin I and II molecules are loaded onto the genome to mediate essential changes in chromosome condensation during mitosis, but it is not clear how the two forms of condensin become distributed on chromosomes. We report here that condensin II, the form of condensin present in the nucleus throughout the cell cycle, is loaded at transcriptionally active promoters, migrates through these genes in a transcription-dependent fashion and accumulates in transcription termination regions during interphase. During mitosis, condensin I is recruited to actively transcribed genes and replaces condensin II. We conclude that the two forms of condensin are loaded at different times during the cell division cycle at the promoters of actively transcribed genes. Overall design: ChIP-Seq data for Condensin II in v6.5 ESCs treated or not with nocodazole

脊椎动物凝缩蛋白I（condensin I）与凝缩蛋白II（condensin II）可被招募装载至基因组，以介导有丝分裂过程中染色体凝缩的关键变化，但目前尚不明确两种凝缩蛋白如何在染色体上实现分布。本研究发现，在整个细胞周期中均定位于细胞核内的凝缩蛋白II，会结合至转录活跃的启动子区域，以转录依赖的方式沿这些基因移动，并在间期积累于转录终止区域。在有丝分裂阶段，凝缩蛋白I会被招募至活跃转录的基因区域，并替换凝缩蛋白II。综上，本研究认为两种凝缩蛋白会在细胞周期的不同时段，于活跃转录基因的启动子区域完成装载。实验整体设计：针对经诺考达唑处理与未处理的v6.5小鼠胚胎干细胞（ESCs）的凝缩蛋白II的染色质免疫共沉淀测序（ChIP-Seq）数据集。