Transcriptome profile of liver, adipose tissue, muscle and PBMC in genetically fat and lean chicken lines submitted to high-fiber and high-fat diet versus standard high-starch diet [PBMC]

The aim of this study was to evaluate the responses of meat-type chicken to changes in dietary energy sources by comparing high-fiber high-fat vs. standard high-starch diets and using a transcriptomic approach in three major metabolic tissues - liver, adipose tissue and muscle - as well as in circulating blood cells. 48 broilers from two experimental lines divergently selected on abdominal fat content (fat and lean lines) were fed with two different diets (high-fiber and high-fat diet (HF) versus standard high-starch (LF) diet).

本研究旨在通过对比高纤维高脂日粮与标准高淀粉日粮，并针对肝脏、脂肪组织、肌肉三大主要代谢组织以及循环血细胞采用转录组学方法（transcriptomic approach），解析肉用鸡对膳食能量来源改变的应答反应。实验选取48只肉用仔鸡，其来源于两个以腹部脂肪含量为选育目标的分化实验品系（肥系与瘦系），并为其饲喂两种不同日粮：高纤维高脂日粮（HF）与标准高淀粉日粮（LF）。