Wild and Landrace Helianthus whole genome sequencing

This project has involved the whole-genome shotgun re-sequencing of wild and landrace sunflower (genus Helianthus) samples to determine patterns of genomic variation. Wild species. DNA was extracted from tissue samples and individually indexed libraries were constructed using Illumina TruSeq LT Sample Prep Kits. Paired-end (2 x 100 bp) sequences were then produced on an Illumina HiSeq 2000 sequencing platform. The resulting data was used for evolutionary analysis of the Helianthus annuus pan-genome.

本项目针对野生及地方品种向日葵（向日葵属*Helianthus*）开展全基因组鸟枪法重测序（whole-genome shotgun re-sequencing），以解析其基因组变异模式。针对野生种，研究人员从组织样本中提取DNA，并采用Illumina TruSeq LT样本制备试剂盒构建单样本索引文库（individually indexed libraries）；随后在Illumina HiSeq 2000测序平台上生成双端（2×100 bp）测序序列。所得测序数据被用于普通向日葵（*Helianthus annuus*）泛基因组（pan-genome）的进化分析。