pAdEasy backbone vectors for production of recombinant adenovirus.

1The backbone plasmids without the adenoviral E3 region are intended for production of vectors for genetic vaccine/gene delivery, while the E3-containing plasmids are more suitable for oncolytic virus development.2A Tat-PTD sequence was inserted in hypervariable region 5 of the adenovirus hexon.3The fiber (shaft and knob) was replaced with the corresponding sequences from adenovirus serotype 35.1. 不含腺病毒E3区域的骨架质粒可用于制备基因疫苗/基因递送载体，而携带E3区域的质粒则更适用于溶瘤病毒的开发。 2. 一段Tat蛋白转导结构域（Tat-PTD）序列被插入至腺病毒六邻体蛋白的高变区5中。 3. 腺病毒纤突蛋白（杆状区与头部区）被替换为来自腺病毒血清型35的对应序列。