Expression data from WT and rtcR, rtcB, and rsr mutant strains of Salmonella enterica serovar Typhimurium strain 14028S with and without 3mM mitomycin C

We have shown that mitomycin C induces expression of the rsr-yrlBA-rtcBA operon in Salmonella Typhimurium in a manner that is dependent on the activator for this operon, RtcR. As such, treatment with mitomycin C generates the ligand or other cellular conditions that activate RtcR and likely generates the substrates on which the components of this RNA repair operon act. We performed an RNA-seq analysis on WT cells and cells with components of the RNA repair operon, or the activator of the operon, deleted with and without mitomycin C. Changes in the transcriptome during mitomycin C between cells that do and do not express the RNA repair system were assessed.

本研究证实，丝裂霉素C（mitomycin C）可通过依赖该操纵子激活因子RtcR的方式，诱导鼠伤寒沙门氏菌（Salmonella Typhimurium）中rsr-yrlBA-rtcBA操纵子的表达。据此，丝裂霉素C处理可产生激活RtcR的配体或其他细胞内调控条件，且大概率生成该RNA修复操纵子各组分所作用的底物。本研究对野生型（WT）细胞、缺失RNA修复操纵子组分或该操纵子激活因子的细胞，分别在有无丝裂霉素C处理的条件下开展了RNA测序（RNA-seq）分析。本研究还评估了经丝裂霉素C处理后，表达与不表达RNA修复系统的细胞之间的转录组变化。