SH1040682.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、交流以及处理基于DNA的物种假说（DNA-based Species Hypotheses，SH）。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一系列动态距离阈值（0.5%至3.0%），被聚类至近似物种级别的单元。所有物种假说都会被赋予唯一且稳定的名称，格式为数字对象标识符（DOI），其分类学与生态学注释将通过分布式的网络第三方注释工作完成验证。系统会综合考量该物种假说下所有序列的鉴定结果，尽可能将物种假说关联至对应的分类单元名称及其所属分类层级（如门、纲、目等）。随后会选取一条自动或人工指定的序列，作为每个物种假说的代表序列。上述代表序列已发布于https://unite.ut.ee/repository.php，可供科学界用于本地序列相似性检索、高通量测序分析流程等研究场景。随着公共真菌ITS序列库的规模持续增长，该系统与数据集将自动完成更新。