Esophageal epithelium of 12 weeks old mice (WT, Nrf2-/-) on C57BL background with or without gastroesophageal reflux for 4 weeks.. Mus musculus

Transcriptional profiling of adult esophageal epithelium comparing wild-type mice with Nrf2-/- mice with or without gastroesophageal reflux for 4 weeks. Goal was to determine the role of Nrf2 on the barrier function of mouse esophageal epithelium. Overall design: Two-class comparisons. Wild-type/without reflux vs. Nrf2-/-/without reflux; Wild-type/gastric reflux vs. Nrf2-/-/gastric reflux; Wild-type/duodenal reflux vs. Nrf2-/-/duodenal reflux; Wild-type/mixed reflux vs. Nrf2-/-/mixed reflux. Biological replicates: 3 replicates for each group.

本数据集针对成年小鼠食管上皮开展转录组表达谱分析，对比经4周有或无胃食管反流处理的野生型小鼠与Nrf2基因敲除（Nrf2-/-）小鼠。本研究旨在明确Nrf2在小鼠食管上皮屏障功能中的调控作用。
实验整体设计包含四组二分类对照：
1. 野生型（无反流）组 vs Nrf2-/-（无反流）组；
2. 野生型（胃反流）组 vs Nrf2-/-（胃反流）组；
3. 野生型（十二指肠反流）组 vs Nrf2-/-（十二指肠反流）组；
4. 野生型（混合反流）组 vs Nrf2-/-（混合反流）组。
每组设置3个生物学重复。