PMACS-108

<b>Project title</b>: PMACS-108<b>Grant Information:</b> AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarction<b>Relevant Citations:</b><b> </b><b>https://doi.org/10.1016/j.isci.2024.110244</b><b>Project Description:</b>· Animal Model: Myocardial Infarction· Animal Age: 8-13 Weeks· Animal Sex: Male· Animal Strain: C57B6/J Lys^CreGATA6^fl/flor C57B6/J Lys^Cre· Number: 9 Mice· Groups: 5 Lys^CreGATA6^fl/fl, 4 Lys^Cre, all infarcted· Experimental Conditions: Mice were given MI via LAD ligation. Animals were sacrificed at day 28 post-infarction. Cardiac function was evaluated via EKG and Echocardiography. Hearts were examined via Mason’s Trichrome histology<b>Code/Scripts: MATLAB </b><b>CODE ATTACHED</b><br><b>Licenses: </b>N/A

<b>项目标题</b>: PMACS-108<b>资助信息</b>: 美国心脏协会（AHA）资助项目9TPA34910007，课题主题为「心包巨噬细胞在心肌梗死（Myocardial Infarction, MI）中的调控作用」<b>相关引用文献</b><b> </b><b>https://doi.org/10.1016/j.isci.2024.110244</b><b>项目描述</b>:· 动物模型：心肌梗死模型· 动物周龄：8~13周· 动物性别：雄性· 动物品系：C57B6/J Lys^CreGATA6^fl/fl 或 C57B6/J Lys^Cre· 实验动物数量：9只小鼠· 分组情况：5只为Lys^CreGATA6^fl/fl基因型，4只为Lys^Cre基因型，所有小鼠均接受心肌梗死造模· 实验方案：通过左前降支（Left Anterior Descending, LAD）结扎构建心肌梗死模型，于造模后第28天处死实验动物。采用心电图（Electrocardiogram, EKG）与超声心动图（Echocardiography）评估心脏功能，通过马松三色（Mason’s Trichrome）组织学染色对心脏组织进行检测<b>代码/脚本</b>: MATLAB，已附带代码<br><b>许可信息</b>: 无