The number of offspring produced by female Drosophila simulans over 4 days following artificial transinfection of Spiroplasma, compared to uninfected controls.

<b>Description of data</b>: The dataset is a record of the number of adult offspring produced by F2 Spiroplasma-transinfected and -uninfected females. For each day there is a record of the number of males and females that emerged, followed by the total number of flies emerged. The D. simulans strain used was F15, which is infected with Wolbachia. Data is in xlsl format. <br><br><b>Description of methods for data collection</b>: Microinjections were carried out as described by Nakayama et al, 2015. The number of adult offspring produced by the F2 generation of infected parental females was counted. Each F2 female was placed with two uninfected males in a vial of fly medium to mate. They were moved into fresh vials every day for five days to allow continuous oviposition. On the sixth day, adult flies were removed, and the females were screened for Spiroplasma. The eclosed offspring from days 2 to 5 were counted. <br><br><b>Column headings</b>:<br><b>Column A</b>- Group: Independent D. simulans lines with alternative nomenclature (a, b, c and d) for easy ordering in R.<br><b>Column B</b>- Line: Independent D. simulans lines (b, c1 and c2) derived from successfully transinfected females and an uninfected control (control).<br><b>Column C</b>- Infection.status: Spiroplasma infection status of D. simulans. Infected (I) and not infected (NI).<br><b>Column D</b>- Block: Spiroplasma infection status of D. simulans. Spiroplasma positive (S+) and Spiroplasma negative (S-).<br><b>Column E</b>- Genotype: lineage (g, i, j, k, c, e, b, d, h and f) of F2 mothers.<br><b>Column F</b>- Rep: replicate female F2 D. melanogaster<br><b>Column G</b>- D2M: adult males from the second day of the fecundity assay<br><b>Column H</b>- D2F: adult females from the second day of the fecundity assay<br><b>Column I</b>- D2T: total number of males and females from the second day of the fecundity assay<br><b>Column J</b>- D3M: adult males from the third day of the fecundity assay<br><b>Column K</b>- D3F: adult females from the third day of the fecundity assay<br><b>Column L</b>- D3T: total number of males and females from the third day of the fecundity assay <br><b>Column M</b>- D4M: adult males from the fourth day of the fecundity assay<br><b>Column N</b>- D4F: adult females from the fourth day of the fecundity assay <br><b>Column O</b>- D4T: total number of males and females from the fourth day of the fecundity assay<br><b>Column P</b>- D5M: adult males from the fifth day of the fecundity assay <br><b>Column Q</b>- D5F: adult females from the fifth day of the fecundity assay<b>Total</b>: total number of adult flies produced from each female Spiroplasma-infected and -uninfected D. simulans.<br><br>

**数据集说明**：本数据集记录了经螺原体（Spiroplasma）转染与未转染的子二代雌蝇所产生的成虫子代数量。每日记录当日羽化的雄蝇、雌蝇数量，随后统计当日总羽化蝇数。所用的拟暗果蝇（D. simulans）品系为F15，该品系感染有沃尔巴克氏体（Wolbachia）。数据以xlsl格式存储。

**数据收集方法说明**：显微注射操作参照Nakayama等人2015年的研究方法进行。统计感染亲代雌蝇的子二代所产生的成虫子代数量。将每头子二代雌蝇与两只未感染的雄蝇置于装有果蝇培养基的试管中进行交配。随后连续5天每日将雌蝇转移至新鲜培养基试管中，以保证持续产卵。于第6天移除成虫，并对雌蝇进行螺原体（Spiroplasma）感染情况筛查。统计第2至5天羽化的子代果蝇数量。

**列标题说明**：
**列A** - 分组（Group）：用于在R语言中便捷排序的独立拟暗果蝇（D. simulans）品系，采用替代命名法（a、b、c、d）。
**列B** - 品系（Line）：源自成功感染转染雌蝇的独立拟暗果蝇（D. simulans）品系（b、c1、c2）以及未感染对照组（control）。
**列C** - 感染状态（Infection.status）：拟暗果蝇（D. simulans）的螺原体（Spiroplasma）感染情况，分为感染组（I）与未感染组（NI）。
**列D** - 区组（Block）：拟暗果蝇（D. simulans）的螺原体（Spiroplasma）感染状态，分为螺原体阳性（S+）与螺原体阴性（S-）。
**列E** - 基因型（Genotype）：子二代雌蝇的谱系（g、i、j、k、c、e、b、d、h、f）。
**列F** - 重复样本（Rep）：子二代黑腹果蝇（D. melanogaster）雌蝇的重复样本。
**列G** - D2M：繁殖力检测第2天的成虫雄蝇数量。
**列H** - D2F：繁殖力检测第2天的成虫雌蝇数量。
**列I** - D2T：繁殖力检测第2天的成虫总数量（雄蝇+雌蝇）。
**列J** - D3M：繁殖力检测第3天的成虫雄蝇数量。
**列K** - D3F：繁殖力检测第3天的成虫雌蝇数量。
**列L** - D3T：繁殖力检测第3天的成虫总数量。
**列M** - D4M：繁殖力检测第4天的成虫雄蝇数量。
**列N** - D4F：繁殖力检测第4天的成虫雌蝇数量。
**列O** - D4T：繁殖力检测第4天的成虫总数量。
**列P** - D5M：繁殖力检测第5天的成虫雄蝇数量。
**列Q** - D5F：繁殖力检测第5天的成虫雌蝇数量。
**总计数（Total）**：每头感染或未感染螺原体（Spiroplasma）的拟暗果蝇（D. simulans）雌蝇所产生的成虫子代总数量。