miRNA data from melanoma-derived exosomes and melanoma cell lines

Identify miRNAs enriched or overexpressed in melanoma-derived exosomes compared to melanoma cells. We analyzed the 2,578 human miRNAs located in the array according to two ways. The first one identified the differentially expressed miRNAs between melanoma-derived exosomes and their parent cells. In this way, we found 198 miRNAs up-regulated in melanoma cell lines compared to their exosomes and 206 miRNAs up-regulated in exosomes compared to their donor cells. The second way of analysis identifies the most expressed miRNAs in the melanoma-derived exosomes without assumption about their expression in parent cells. We defined two criteria; the first one is a RMA (Robust Multi-Array Average, a good alternative to gene expression value) above 5, corresponding to the mean expression level of the array and a SD ≤ 0.2 between all samples to discover miRNAs expressed uniformly between melanoma-derived exosomes M113 and M117. We identified 44 miRNAs under these criteria. We used microarray to identified miRNA enriched or overexpressed in melanoma-derived exosomes compared to their parent cells.

本研究旨在筛选相较于黑色素瘤细胞，在黑色素瘤来源外泌体中富集或高表达的微小核糖核酸（miRNAs）。本研究针对芯片中包含的2578个人类miRNAs，采用两种分析方案开展研究：第一种方案用于鉴定黑色素瘤来源外泌体与其亲本细胞间的差异表达miRNAs。通过该方案，我们发现相较于对应外泌体，黑色素瘤细胞系中有198个miRNAs呈上调表达；而相较于其供体细胞，外泌体中则有206个miRNAs呈上调表达。第二种分析方案无需预先假定miRNAs在亲本细胞中的表达水平，仅用于筛选黑色素瘤来源外泌体中的高表达miRNAs。我们设定了两项筛选标准：其一为RMA（稳健多阵列平均法（Robust Multi-Array Average），基因表达值分析的优质替代方案）数值大于5，该数值对应芯片的平均表达水平；其二为所有样本间的标准差（SD）≤0.2，以此筛选在黑色素瘤来源外泌体M113与M117中表达均一的miRNAs。通过上述标准，我们共鉴定得到44个符合要求的miRNAs。本研究通过微阵列芯片技术，筛选得到相较于亲本细胞在黑色素瘤来源外泌体中富集或高表达的miRNAs。