Impact of Experimental Variables on Methylation in Populus nigra through Targeted Genome Bisulfite Sequencing.

We investigated the impact of several experimental conditions on the methylation level of a single individual following custom targeted genome bisulfite sequencing. Impacts of DNA fragmentation, input DNA quantity, probe dilution and the number of PCR cycles were quantified by comparing the distribution of methylation over 18Kb genomic sequence in the same individual.

本研究针对单一个体，采用定制靶向基因组亚硫酸氢盐测序（custom targeted genome bisulfite sequencing）技术，探究了多种实验条件对其基因组甲基化水平的影响。通过比对该个体18Kb基因组序列的甲基化分布情况，量化了DNA片段化程度、起始DNA投入量、探针稀释度以及PCR循环次数的影响效应。