Hierarchal gene master regulators of adenocarcinomic human alveolar basal epithelial cells A549.

In previous papers we proved that cancer nodules and surrounding normal tissue are governed by different gene master regulators (GMRs) and that expression manipulation of a cancer GMR can selectively destroy cancer cells with minimal effects on the normal ones. GMR is defined as a coding or non-coding transcript whose strictly controled abundance by the cell homeostatic mechanisms regulates most major cell functional pathways. Here, we determined the gene hierarchy in the standard adenocarcinomic human alveolar basal epithelial cell line A549. One condition, 4 replicas

既往研究已证实，癌结节与周围正常组织受不同的基因主调控因子（gene master regulators，GMRs）调控，且对癌性基因主调控因子进行表达操作可选择性杀伤癌细胞，对正常细胞的影响极小。基因主调控因子指受细胞稳态机制严格调控其丰度、并可调控绝大多数核心细胞功能通路的编码或非编码转录本。本研究解析了标准人肺泡基底上皮腺癌细胞系A549中的基因层级结构。本实验设置1组处理条件，包含4个生物学重复。