Understanding how H1 modifications influence chromatin accessibility around DSBs, to elucidate the underlying mechanisms.

For H1 PTM identification, HeLa cells were exposed to 10 Gy IR or not and released for 2 min. Histones were extracted and deamidated peptides were identified by mass spectrometry (MS). For CTPS1 phosphorylation sites identification, whole cell lysates were extracted, immunoprecipitated using anti-FLAG, and analyzed by MS.

针对组蛋白H1翻译后修饰（Post-translational modification, PTM）的鉴定实验：将海拉细胞施加或不施加10戈瑞电离辐射（Ionizing Radiation, IR），辐照后恢复培养2分钟；随后提取组蛋白，通过质谱（Mass Spectrometry, MS）鉴定去酰胺化肽段。针对CTPS1磷酸化位点的鉴定实验：提取全细胞裂解液，使用抗FLAG标签抗体进行免疫共沉淀，随后通过质谱分析。