Dubuis et al. - Raw_Profiles
收藏DataONE2013-01-22 更新2024-06-27 收录
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Each file contains the source data for raw gap gene (Hb, Kr, Gt, Kni) dorsal intensity profiles measured using immunofluorescence techniques in 163 Drosophila embryos during n.c. 14. Column 1 contains the embryo number referring to its position on the slide (see Images.zip). Column 2 contains an information about the azimuthal orientation of the embryo on the slide ('1' if the confocal plane is closer to midsagittal plane, '2' if it is closer to the coronal plane). Column 3 contains the furrow canal depth (delta_FC) measured in micrometers. Column 4 contains the corresponding estimated age in minutes. Columns 5 to 1004 contain a 1x1000 vector representing the dorsal intensity profile of the gap gene (Hb, Kr, Gt, Kni) in the embryo. The 1000 points are equally spaced along the AP axis. Thus, Intensity345 represents the intensity at 34.5%EL. NaN means that we couldn't reliably detect the profile intensity at that position (usually near the edges). Columns 1-4 are identical in the four files.
每个文件均包含163个果蝇(Drosophila)胚胎在核分裂周期14(nuclear cycle 14,n.c. 14)阶段,通过免疫荧光技术(immunofluorescence techniques)测得的间隙基因(gap gene)Hb、Kr、Gt、Kni的背侧强度分布原始数据。第1列为胚胎编号,对应其在载玻片上的位置(详见Images.zip)。第2列记录胚胎在载玻片上的方位朝向:若共焦平面(confocal plane)更接近正中矢状面(midsagittal plane)则记为'1',若更接近冠状面(coronal plane)则记为'2'。第3列为以微米(micrometers,μm)为单位测得的沟管深度(delta_FC)。第4列为对应的预估发育时长(单位:分钟)。第5列至第1004列为1×1000的向量,代表该胚胎间隙基因(Hb、Kr、Gt、Kni)的背侧强度分布,该1000个采样点沿前后轴(Anterior-Posterior axis,AP轴)等间距分布。因此,Intensity345代表胚胎长度(Egg Length,EL)34.5%位置处的信号强度。NaN表示无法在该位置(通常位于胚胎边缘附近)可靠检测到强度分布信号。四个文件的第1至第4列内容完全一致。
创建时间:
2013-01-22



