Phenotypic analysis of monocytes and MDDC.

PBMCs were isolated from fresh donor blood buffy coats. After an aggregation step, the cells were cultured with or without 25 ng/ml IL-4 and 50 ng/ml GM-CSF for 5 days to derive MDDC or monocytes, respectively. Expression of several cell surface markers was analyzed flow cytometrically with specific PE-labeled mAbs. Data represent mean % positive cells ± standard error of the mean (SEM) from 5 different donors. The expression level of each marker was compared between monocytes and MDDC and the corresponding p-value was calculated with a paired t-test.从新鲜捐献者血液的血沉棕黄层中分离外周血单个核细胞（PBMCs）。经聚集步骤处理后，将细胞分别在添加或不添加25 ng/ml白细胞介素4（IL-4）与50 ng/ml粒细胞-巨噬细胞集落刺激因子（GM-CSF）的培养基中培养5天，以分别诱导获得单核细胞来源树突状细胞（MDDC）与单核细胞。采用特异性PE标记单克隆抗体（mAbs），通过流式细胞术对多种细胞表面标志物的表达水平进行检测。数据来源于5名不同捐献者，以阳性细胞百分比均值±均值标准误（standard error of the mean, SEM）呈现。对单核细胞与单核细胞来源树突状细胞的各标志物表达水平进行组间比较，并通过配对t检验计算对应的p值。